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  1. #1
    Shut up and squat ! g1234's Avatar
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    Best way to sterilize gear?
    Below are several methods i collected from the web (most from Animal's website). What do you guys think? Which method would you use?

    Method1: Filtering and BA
    Filter the gear with a .2 micron filter and add 3-5% BA to the gear

    Method2: Microwave Sterilization
    This is the easiest method to use and fastest.


    step 2 - Nothing should be covering the top of the vial. Take the seal and the
    stopper off the top of the vial to where the oil is totally exposed. You can
    do this with a pair of pliers or some instrument that can pry the lid off. The
    seal will pry off pretty easily. I use a tool that is on my pocket knife. It
    resembles a can opener.

    step 3 - Place the vial in the microwave. Make sure there is nothing covering
    the opening of the vial. Put the microwave on 20 seconds and hit start. After
    it is through let sit for about 5 minutes and then repeat 2 more times.

    step 4 - After the 3rd time immediately take the vial out of the microwave.
    You may need mittens because it WILL be hot. At this point you should preload
    all your syringes while the liquid is still hot. This is my preferred method
    because it keeps everything sterile. Make sure to use 2 needles per syringe. 1
    should be used to draw up the fluid. The other should be a clean one that will
    be injected in your ass. The top of the second one should NOT BE REMOVED until
    the time of injection. This ensures total sterility. If you do not want to
    preload your syringes then youw ill need to put the rubber stopper back on the
    syringe. Before you do that though plop it in the microwave for about 20
    seconds on a paper towl or plate. Immediately take the stopper out and put it
    on the vial using the napkin to pick it up with. This is a pain in the ass
    though when the time comes to draw up the fluid. Not only do you expose the
    oil once again when you take the seal off but you risk spilling the oil all
    over the place. It is just easier to preload all the syringes when the oil is
    still hot and sterile. Then you dont have to mess with it at a later date and
    you can just inject

    NOTE: I will be developing oil products soon. I will be using these same
    techniques to sterilize the oils I will be selling. The vials will be
    professionally sealed and will be ready to withdraw the fluid without first

    Method3: Boil
    This takes some time cause you have to bring the water to a boil and then put
    the vial in there. If you do not want to expose the oil because you will not
    be preloading then I would use this method or the bake method.

    step 1 - Stick a needle from a syringe through the rubber top of the vial so
    that it is sticking through it by about a half an inch. The purpose of this is
    to allow the BA to evaporate out of the vial. If it does not then the vial

    step 2 - Place the vial in a small pot. Fill the water level up to about a
    half an inch below the top of the vial. Turn on the stovetop to about medium
    high. You can even do high. (I do) Wait till the water starts boiling before
    you start counting down the minutes. Let the vial sit in the water for 15
    minutes then remove. (the vial may move around in the water as the bubbles
    will push it around but it will not fall over)

    step 3 - Sit the vial on a paper towl or other surface that can soak of any
    water. Take the needle out of the top of the vial before it cools. If you let
    it cool the needle will most likely get stuck there. It is actually smart to
    leave it in there though. This way you can just put the syringe on needle and
    draw up the fluid as you go. But if you do this make sure to put another
    syringe on there when you are through with filling the other one. If you dont
    you are leaving a small hole open to let bacteria get in. That is it for the
    boil method. You can preload the syringes if you like after you take the vial
    out. Beware though. It is a pain in the ass to attempt to preload the syringes
    with the top on there and the liquid is still hot cause you will have to turn
    it upside down to get the last bit of liquid in the syringes. That vial is
    very hot!

    Method 4: Bake Method
    Bakind is pretty much the same procedure as the boil method. Just place the
    vial in a pan and put a needle in the top. Bake 15 minutes on 250 degrees.
    Wait about 10-15 min and repeat 2 more times.

    I hope this helps some of you guys that may be weary about ordering IP gear
    and other vet products. All oils CAN be heat sterilized. If you take the
    correct procedures you will have IMO human grade products after you heat
    sterilize the product. Besides....test testosterone propionate is test testosterone propionate after all. It doesnt
    matter if a pharmaceutical company produces it or IP. As long as the dosages
    are correct it works the same way in your body. The only difference is human
    grade is sterile shipped to you and IP is not always sterile. It is just smart
    to heat sterilize it. For the money you cannot beat private lab made gear, and
    if proper sterilization processes are use it is the same thing as human grade.

    If your oil is shipped to you in a plastic container of some sort you cannot
    obviously put this in boiling water or the oven. You can however put this in
    the microwave for 20 seconds. If it is in a plastic container you must let the
    product totally cool though before reheating it 2 more times in the microwave
    or you risk the plastic vial melting.

  2. #2
    - FerrariF50's Avatar
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    method 1 is the only method listed which might achieve sterilization

    the rest are wrong and inadequate

  3. #3
    Shut up and squat ! g1234's Avatar
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    F50 .2 micron filter is a good choice for that? i've seen some people use .45 - .5

    Whats your reason for saying the other methods are wrong?

  4. #4
    - FerrariF50's Avatar
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    0.2 µm will filter cleaner than 0.45 µm
    generally 0.2 µm is the largest pore size accepted to produce a sterile filtrate

    methods 2, 3 and 4 revolve around the basis of heat

    water boils at 100 degrees celsius which is insufficient to achieve sterilization.
    although there is a method to sterilize using boiling water, its not the one above

    dry heat sterilization is achieved at 160 degrees celsius for at least 2 hours which renders methods 2 and 4 useless (possibly more for oils)

    also note for method 1 to work, the gear would need to be filtered directly into a sterile vial using a sterile filter housing and membrane and that BA is not used to achieve sterilization, but to preserve it

  5. #5
    Shut up and squat ! g1234's Avatar
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    So other methods could work but with higher healing temperatures? Why are they so well speard?

    What about microwave oven ?

  6. #6
    A Legend GymLift's Avatar
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    May 2004
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    Sorry guys, but I need to close this thread because it touches on a sensitive area that we can't allow.

    All that I'll say in closing is that the posted article is full of a lot of bullshit.

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